Development of novel optically encoded microspheres for microarrays

Vladimir A. Sabetsky; Serguei V. Martiouchine; Zinaida V. Nesterova

doi:10.1117/12.472065

21 June 2002 Development of novel optically encoded microspheres for microarrays

Vladimir A. Sabetsky, Serguei V. Martiouchine, Zinaida V. Nesterova

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472065
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The use of DNA microarrays for analysis of complex biological samples is becoming a mainstream part of biomedical research. Microarrays and gene chips containing a lot of specific polynucleotide sequence of both and novel, can be used to establish the expression of numerous genes in a single experiment. We developed a multicolor fluorescent microbeads for differential display method that combines properties of both differential signal amplification and DNA microarray in signal analysis. The multicolor fluorescent differential display provides a basis upon which a sensitive and accurate gene expression process can be automated and digitally analyzed. The ability to perform such analysis for extremely large nu8mbers of probes on an inexpensive and well-defined high-throughput platform is highly desirable. Microbead-based libraries are emerging as a very attractive alternative smaller probe sites. Most impressive advantages of microbeads are that they inexpensive to produce in a large numbers, they can be conveniently stored in a small volume of fluid, and they can be easily bar coded and screened using a variety of technologies. We developed a dextran-based microbeads containing covalently coupled fluorescent compounds. By combination of different compounds attached to dextran molecules it was possible to increase the number of 'fluorescently barcoded beads'.

Citation Download Citation

Vladimir A. Sabetsky, Serguei V. Martiouchine, and Zinaida V. Nesterova "Development of novel optically encoded microspheres for microarrays", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472065

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