Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

High-Q whispering-gallery mode sensor in liquids

[+] Author Affiliations
Jay L. Nadeau, Vladimir S. Ilchenko, Gregory H. Bearman, Lute Maleki

Jet Propulsion Lab. (USA)

Dmitri Kossakovski

Oewaves, Inc. (USA)

Proc. SPIE 4629, Laser Resonators and Beam Control V, 172 (June 5, 2002); doi:10.1117/12.469488
Text Size: A A A
From Conference Volume 4629

  • Laser Resonators and Beam Control V
  • Alexis V. Kudryashov
  • San Jose, CA | January 18, 2002

abstract

Optical sensing of biomolecules on microfabricated glass surfaces requires surface coatings that minimize nonspecific binding while preserving the optical properties of the sensor. Microspheres with whispering-gallery (WG) modes can achieve quality factor (Q) levels many orders of magnitude greater than those of other WG-based microsensors: greater than 1010 in air, and greater than 109 in a variety of solvents, including methanol, H2O and phosphate buffered saline (PBS). The presence of dyes that absorb in the wavelength of the WG excitation in the evanescent zone can cause this Q value to drop by almost 3 orders of magnitude. Silanization of the surface with mercapto-terminal silanes is compatible with high Q (>109), but chemical cross-linking of streptavidin reduces the Q to 105-106 due to build-up of a thick, irregular layer of protein. However, linkage of biotin to the silane terminus preserves the Q at a ~2x107 and yields a reactive surface sensitive to avidin-containing ligands in a concentration-dependent manner. Improvements in the reliability of the surface chemistry show promise for construction of an ultrasensitive biosensor.

© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Jay L. Nadeau ; Vladimir S. Ilchenko ; Dmitri Kossakovski ; Gregory H. Bearman and Lute Maleki
"High-Q whispering-gallery mode sensor in liquids", Proc. SPIE 4629, Laser Resonators and Beam Control V, 172 (June 5, 2002); doi:10.1117/12.469488; http://dx.doi.org/10.1117/12.469488


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.