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Proceedings Article

Time-gated fluorescence imaging of chloroaluminum phthalocyanine tetrasulfonate in a tissue phantom

[+] Author Affiliations
Sarah L. Gundy, Andrew Shearer, Daniel J. Buckton, Alan G. Ryder, Michael Ball

National Univ. of Ireland, Galway (Ireland)

Wilhelm J. M. van der Putten

Univ. College Hospital Galway (Ireland)

Proc. SPIE 4876, Opto-Ireland 2002: Optics and Photonics Technologies and Applications, 109 (March 17, 2003); doi:10.1117/12.462794
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From Conference Volume 4876

  • Opto-Ireland 2002: Optics and Photonics Technologies and Applications
  • Thomas J. Glynn
  • Galway, Ireland | September 05, 2002

abstract

Phthalocyanine derivatives are currently under investigation for use in Photodynamic Therapy, which is a promising treatment for cancer. These materials, which display preferential uptake in cancerous cells, also exhibit high fluorescence yields, and can be used for tumour detection. Problems with steady-state fluorescence techniques such as background autofluorescence can be eliminated by the use of time-resolved techniques. Improved contrast can be obtained with time-resolved techniques because of the differing lifetimes between endogenous and exogenous photosensitisers. An imaging system was constructed using a fast (200 psec) gated CCD camera and a pulsed 635 nm laser diode. A tissue phantom was assembled to test the system by drilling thirty-six wells of varying diameter and depth (10 mm to 1 mm) into a block of polymethyl methacrylate (PMMA). The system was used to record images of chloroaluminum phthalocyanine tetrasulfonate within the wells at differing concentrations in phosphate buffer. A mixture of 1) Intralipid to mimic tissue scatter, 2) Evans blue to mimic tissue absorption, and 3) zinc phthalocyanine tetrasulfonate to mimic healthy tissue autofluorescence of varying depth was placed on top of the PMMA block. These results contribute to the precision of a time-gated imaging system to image living organisms using fluorescence lifetimes.

© (2003) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Sarah L. Gundy ; Wilhelm J. M. van der Putten ; Andrew Shearer ; Daniel J. Buckton ; Alan G. Ryder, et al.
"Time-gated fluorescence imaging of chloroaluminum phthalocyanine tetrasulfonate in a tissue phantom", Proc. SPIE 4876, Opto-Ireland 2002: Optics and Photonics Technologies and Applications, 109 (March 17, 2003); doi:10.1117/12.462794; http://dx.doi.org/10.1117/12.462794


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