Laser-induced fluorescent spectroscopy of steroid hormones

Elena S. Samoilova; Vyacheslav I. Fedorov; Olga P. Cherkasova; Yuri P. Meshalkin

doi:10.1117/12.475592

16 July 2002 Laser-induced fluorescent spectroscopy of steroid hormones

Elena S. Samoilova, Vyacheslav I. Fedorov, Olga P. Cherkasova, Yuri P. Meshalkin

Proceedings Volume 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III; (2002) https://doi.org/10.1117/12.475592
Event: Saratov Fall Meeting 2001, 2001, Saratov, Russian Federation

Abstract

The laser-induced fluorescence spectra of steroid hormones - estradiol, estriol, estrone, androstenedione - are obtained at excitation of the fourth harmonic of Nd:YAG laser radiation. The quantum yields of fluorescence of these substances were rated by means of the relative method. They are 1.11 X 10^-1, 5.20 X 10^-3, 8.47 X 10^-5. The water solution of tryptophan was used as a standard. The set-up sensitivity for high and average quantum yields substances has been defined.

Citation Download Citation

Elena S. Samoilova, Vyacheslav I. Fedorov, Olga P. Cherkasova, and Yuri P. Meshalkin "Laser-induced fluorescent spectroscopy of steroid hormones", Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, (16 July 2002); https://doi.org/10.1117/12.475592

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