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Proceedings Article

Laser-induced fluorescent spectroscopy of steroid hormones

[+] Author Affiliations
Elena S. Samoilova, Vyacheslav I. Fedorov, Olga P. Cherkasova

Institute of Laser Physics (Russia)

Yuri P. Meshalkin

Institute of Physiology and Novosibirsk State Technical Univ. (Russia)

Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, 315 (July 16, 2002); doi:10.1117/12.475592
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From Conference Volume 4707

  • Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III
  • Valery V. Tuchin
  • Saratov, Russia | October 02, 2001

abstract

The laser-induced fluorescence spectra of steroid hormones - estradiol, estriol, estrone, androstenedione - are obtained at excitation of the fourth harmonic of Nd:YAG laser radiation. The quantum yields of fluorescence of these substances were rated by means of the relative method. They are 1.11 X 10-1, 5.20 X 10-3, 8.47 X 10-5. The water solution of tryptophan was used as a standard. The set-up sensitivity for high and average quantum yields substances has been defined.

© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Elena S. Samoilova ; Vyacheslav I. Fedorov ; Olga P. Cherkasova and Yuri P. Meshalkin
"Laser-induced fluorescent spectroscopy of steroid hormones", Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, 315 (July 16, 2002); doi:10.1117/12.475592; http://dx.doi.org/10.1117/12.475592


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