Lifetime fluorescence spectroscopy for in situ investigation of osteogenic differentiation

Laura Marcu; Amir Elbarbary; Patricia Zuk; Daniel A. De Ugarte; Prosper Benhaim; Hamza Kurt; Marc H. Hedrick; Peter Ashjian

doi:10.1117/12.477913

27 August 2003 Lifetime fluorescence spectroscopy for in situ investigation of osteogenic differentiation

Laura Marcu, Amir Elbarbary, Patricia Zuk, Daniel A. De Ugarte, Prosper Benhaim, Hamza Kurt, Marc H. Hedrick, Peter Ashjian

Author Affiliations +

Proceedings Volume 4961, Laser-Tissue Interaction XIV; (2003) https://doi.org/10.1117/12.477913
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Time-Resolved Laser-Induced Fluorescence Spectroscopy (TR-LIFS) represents a potential tool for the in-situ characterization of bioengineered tissues. In this study, we evaluate the application of TR-LIFS to non-intrusive monitoring of matrix composition during osteogenetic differentiation. Human adipose-derived stem cells, harvested from 3 patients, were induced in osteogenic media for 3, 5, and 7 weeks. Samples were subsequently collected and probed for time-resolved fluorescence emission with a pulsed nitrogen laser. Fluorescence parameters, derived from both spectral- and time-domain, were used for sample characterization. The samples were further analyzed using Western blot analysis and computer-based densitometry. A significant change in the fluorescence parameters was detected for samples beyond 3 weeks of osteogenic differentiation. The spectroscopic observations: 1) show increase of collagen I when contrasted against the time-resolved fluorescence spectra of commercially available collagens; and 2) are in agreement with Western blot analysis that demonstrated significant increase in collagen I content between 3- vs. 5-weeks and 3- vs. 7-weeks and no changes for collagens III, IV, and V. Our results suggest that TR-LIFS can be used as a non-invasive means for the detection of specific collagens in maturing connective tissues.

Citation Download Citation

Laura Marcu, Amir Elbarbary, Patricia Zuk, Daniel A. De Ugarte, Prosper Benhaim, Hamza Kurt, Marc H. Hedrick, and Peter Ashjian "Lifetime fluorescence spectroscopy for in situ investigation of osteogenic differentiation", Proc. SPIE 4961, Laser-Tissue Interaction XIV, (27 August 2003); https://doi.org/10.1117/12.477913

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Collagen

Luminescence

Tissues

Time resolved spectroscopy

Fluorescence spectroscopy

Spectroscopy

Skin

Show All Keywords

Keywords/Phrases

Search In:

Publication Years