Photo-induced flip-flop of amphiphilic membrane markers

Thomas Pons; Jerome Mertz

doi:10.1117/12.478022

10 July 2003 Photo-induced flip-flop of amphiphilic membrane markers

Thomas Pons, Jerome Mertz

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.478022
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

We demonstrate localized photo-induced flip-flop of stilbazolium markers in model lipid bilayer membranes. The flip-flop mechanism and dynamics are determined by combined two-photon excited fluorescence and second harmonic generation microscopy. Upon illumination of labeled membranes with a femtosecond laser beam, two-photon absorption induced photo-isomerization provokes a significant increase in the cis-marker population whose flip-flop rate was determined to be at least a thousand times faster than that for trans-markers.

Citation Download Citation

Thomas Pons and Jerome Mertz "Photo-induced flip-flop of amphiphilic membrane markers", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.478022

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