Benefits and applications of programmable spectral devices in confocal microscopes

Rolf Borlinghaus

doi:10.1117/12.477987

9 July 2003 Benefits and applications of programmable spectral devices in confocal microscopes

Rolf Borlinghaus

Proceedings Volume 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X; (2003) https://doi.org/10.1117/12.477987
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Recent developement in confocal microscope systems allow to tune the spectral characteristics of those elements which separate excitation and emission. The new devices replace the classical filter-devices, which have been used in fluorescence microscopy for many years. Excitation filters have been replaced by acousto optical filters, which work as multichannel dimmer devices and allow fine tuning of excitation energy as well as tuning the excitation ratio of multiple excitations. Emission filters have been replaced by tunable multiband detectors, which allow tuning of emission band width for higher efficiency and better band separation. The multiband detector also allows spectral scanning modes. Dichroitic beam splitters have been replaced by acousto optical beam splitters (AOBS) giving significantly increased signal efficiency and allow fast switching of excitation modes without moving mechanical parts. All three devices together lead to much better primary images, either giving highly improved signal to noise ratio or much less photobleaching (which is identically with viability of life samples).

Citation Download Citation

Rolf Borlinghaus "Benefits and applications of programmable spectral devices in confocal microscopes", Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); https://doi.org/10.1117/12.477987

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