The Hyperion instrument mounted on the EO-1 spacecraft was launched November 21, 2000 into an orbit following LANDSAT-7 by 1 minute. Hyperion has a 7.5 km swath width, a 30 meter ground resolution and 220 spectral bands. Its spectral bands extend from 400 nm to 2500 nm with each band having about a 10 nm bandwidth. A unique process to validate the spectral calibration was developed. The process was based on an atmospheric limb collect and supported with a solar calibration collect. The data contained a collection of solar lines, atmospheric lines and absorption lines from the paint that coats the solar calibration reflectance panel. Correlating the positions of these lines with reference data, the center wavelength of each pixel across the field of view for the VNIR and SWIR spectral regions of the imaging spectrometer has been verified. In this paper we discuss the data collection and the technique applied to the VNIR and SWIR focal plane array.© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.