Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Detection of living cells in stratospheric samples

[+] Author Affiliations
Melanie J. Harris, Nalin C. Wickramasinghe, David Lloyd, Michael P. Turner, Shirwan Al-Mufti, Fred Hoyle

Univ. of Wales Cardiff (United Kingdom)

J. V. Narlikar

Inter-Univ. Ctr. for Astronomy and Astrophysics (India)

P. Rajaratnam

Indian Space Research Organization (India)

Max K. Wallis

Univ. fo Wales Cardiff (United Kingdom)

S. Ramadurai

Tata Institute for Fundamental Research (India)

Proc. SPIE 4495, Instruments, Methods, and Missions for Astrobiology IV, 192 (February 6, 2002); doi:10.1117/12.454758
Text Size: A A A
From Conference Volume 4495

  • Instruments, Methods, and Missions for Astrobiology IV
  • Richard B. Hoover; Gilbert V. Levin; Roland R. Paepe; Alexei Y. Rozanov
  • San Diego, CA, USA | July 29, 2001

abstract

Air samples collected aseptically over tropical India at various stratospheric altitudes ranging from 20 to 41 km using cryosampler assemblies carried on balloons flown from Hyderabad have shown evidence of living microbial cells. Unambiguous evidence of living cells came from examining micropore filters on which the samples were recovered with the use of voltage sensitive lipophilic dyes that could detect the presents of active cells. Clumps of viable cells were found at all altitudes using this technique, and this conclusion was found to be consistent with images obtained from electron microscopy. Since the 41 km sample was collected well above the local tropopause, a prima facie case for a space incidence of these microorganisms is established. Further work on culturing, PCR analysis and isotopic analysis is in progress.

© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Melanie J. Harris ; Nalin C. Wickramasinghe ; David Lloyd ; J. V. Narlikar ; P. Rajaratnam, et al.
"Detection of living cells in stratospheric samples", Proc. SPIE 4495, Instruments, Methods, and Missions for Astrobiology IV, 192 (February 6, 2002); doi:10.1117/12.454758; http://dx.doi.org/10.1117/12.454758


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.