Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Autofluorescence properties of skin and applications in dermatology

[+] Author Affiliations
Haishan Zeng, Calum E. MacAulay

BC Cancer Agency (Canada)

David I. McLean, Harvey Lui

Univ. of British Columbia and Vancouver Hospital and Health Sciences Ctr. (Canada)

Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, 366 (October 11, 2000); doi:10.1117/12.403940
Text Size: A A A
From Conference Volume 4224

  • Biomedical Photonics and Optoelectronic Imaging
  • Hong Liu; Qingming Luo
  • Beijing, China | November 08, 2000

abstract

Skin autofluorescence was observed as early as 1908. Its applications in dermatology was first reported in 1925- the use of Wood's lamp for the detection of fungal infection. In the first part of the paper, a historical review was presented on skin autofluorescence properties. In the second part, systematic research done in out laboratory on autofluorescence properties of normal and diseased skin was summarized. We developed three tools for the study: 1) a compact fiber optic spectrometer for in vivo macroscopic fluorescence spectral measurements on volunteers and patients; 2) a CCD camera based fluorescence imaging for in vivo macroscopic imaging of 2D fluorescence intensity distributions over various skin diseases; 3) a fiber optic microspectrophotometer (MSP) system for in vitro microscopic fluorescence spectral measurements and fluorescence imaging of frozen tissue sections. With these tools, we obtained the excitation-emission matrices (EEMs) of in vivo normal skin, the temporal dynamics of skin autofluorescence decay under continuous wave laser exposure, and fluorescence spectra of 1500 lesions from 600 patients spanning 35 disease types. Monte Carlo simulation has been employed to explain the autofluorescence decay dynamics and to reconstruct the in vivo spectra from in vitro microscopic fluorophore distribution and intrinsic fluorescence spectra of various skin structures. Spectral feature based linear discrimination function analysis and principal components decomposition analysis are performed to assess the potential of autofluorescence spectroscopy for skin cancer detection. Clinical test of a fluorescence scope system for skin cancer margin delineation is under way.

© (2000) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Haishan Zeng ; David I. McLean ; Calum E. MacAulay and Harvey Lui
"Autofluorescence properties of skin and applications in dermatology", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, 366 (October 11, 2000); doi:10.1117/12.403940; http://dx.doi.org/10.1117/12.403940


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

 

 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.