Integrated instrument for dynamic light scattering and natural fluorescence measurements

Luigi Rovati; Luca Pollonini; Rafat R. Ansari

doi:10.1117/12.429271

7 June 2001 Integrated instrument for dynamic light scattering and natural fluorescence measurements

Luigi Rovati, Luca Pollonini, Rafat R. Ansari

Proceedings Volume 4245, Ophthalmic Technologies XI; (2001) https://doi.org/10.1117/12.429271
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Over the past two decades, great efforts have been made in ophthalmology to use optical techniques based on dynamic light scattering and tissue natural fluorescence for early (at molecular level) diagnosis of ocular pathologies. In our previous studies, the relationship between the corneal AF and DLS decay widths of ocular tissues were established by performing measurements on diabetes mellitus patients. In those studies, corneal AF mean intensities were significantly correlated with DLS decay width measurements for each diabetic retinopathy grade in the vitreous and in the cornea. This suggested that the quality of the diagnosis could be significantly improved by properly combining these two powerful techniques into a single instrument. Our approach is based on modifying a commercial scanning ocular fluorometer (Fluorotron Master, Ocumetrics Inc., CA, USA) to include both techniques in the same scanning unit. This configuration provides both DLS and AF real time measurements from the same ocular volume: they can be located in each section of the optical axis of the eye from the cornea to the retina. In this paper, the optical setup of the new system is described and preliminary in-vitro and in-vivo measurements are presented.

Citation Download Citation

Luigi Rovati, Luca Pollonini, and Rafat R. Ansari "Integrated instrument for dynamic light scattering and natural fluorescence measurements", Proc. SPIE 4245, Ophthalmic Technologies XI, (7 June 2001); https://doi.org/10.1117/12.429271

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