CO2 laser biopsies of oral mucosa: an immunocytological and histological comparative study

Marina Consuelo Vitale; Annibale Renzo Botticelli; Davide Zaffe; Alessandra Martignone; Aurelia Cisternino; Franco Vezzoni; Francesco Scarpelli

doi:10.1117/12.424491

27 April 2001 CO₂ laser biopsies of oral mucosa: an immunocytological and histological comparative study

Marina Consuelo Vitale, Annibale Renzo Botticelli, Davide Zaffe, Alessandra Martignone, Aurelia Cisternino, Franco Vezzoni, Francesco Scarpelli

Author Affiliations +

Proceedings Volume 4249, Lasers in Dentistry VII; (2001) https://doi.org/10.1117/12.424491
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The relationship between bioptic technique and tissue preservation has been studied in 18 oral biopsies of young patients obtained by electro surgery or CO₂ laser surgery. Biopsies were formalin fixed, paraffin embedded and histologically, histochemically and immunocytochemically treated. All the biopsies show inflammatory cell infiltration, epithelial spongiosis, trichocariosis, supra basal small blisters, and epithelial clefts with lamina detaching from the corium. Histochemistry shows both the presence of edema and acid mucopolysaccharides inside the corium, and variable glycogen content in epithelial cells. Trichocariotic cells show a positive MiB1/Ki67 expression, when they are present. Nevertheless, laser biopsies show a lower amount of basophilic fibrous tissue and of bc12 bodies detection, connected with a higher amount of glycogen, Cytokeratin and MiB1/Ki67 expression in epithelial cells, compared to bovie biopsies. The result show a higher degree of damages in particular at the epithelial level, in electro surgery biopsies rather than laser biopsies. The best epithelial and corium preservation showed by laser biopsies suggest a chance of reversible condition, which can lead to a complete recovery due to its higher capability of restoring tissues.

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Marina Consuelo Vitale, Annibale Renzo Botticelli, Davide Zaffe, Alessandra Martignone, Aurelia Cisternino, Franco Vezzoni, and Francesco Scarpelli "CO₂ laser biopsies of oral mucosa: an immunocytological and histological comparative study", Proc. SPIE 4249, Lasers in Dentistry VII, (27 April 2001); https://doi.org/10.1117/12.424491

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