3D imaging of lung tissue by confocal microscopy and micro-CT

Andres Kriete; Andreas Breithecker; Wigbert D. Rau

doi:10.1117/12.434736

9 July 2001 3D imaging of lung tissue by confocal microscopy and micro-CT

Andres Kriete, Andreas Breithecker, Wigbert D. Rau

Proceedings Volume 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical; (2001) https://doi.org/10.1117/12.434736
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Two complementary techniques for the imaging of tissue subunits are discussed. A computer guided light microscopic imaging technique is described first, which confocally resolves thick serial sections axially. The lateral area of interest is increased by scanning a mosaic of images in each plane. Subsequently, all images are fused digitally to form a highly resolved volume exhibiting the fine structure of complete respiratory units of lung. A different technique described is based on microtomography. This method allows to image volumes up to 3x3x3 cm at a resolution of up to 7 microns. Due to the lack of strong density differences, a contrast enhancement procedure is introduced which makes this technique applicable for the imaging of lung tissue. Imaging, visualization and analysis described here are parts of an ongoing project to model structure and to simulate function of tissue subunits and complete organs.

Citation Download Citation

Andres Kriete, Andreas Breithecker, and Wigbert D. Rau "3D imaging of lung tissue by confocal microscopy and micro-CT", Proc. SPIE 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, (9 July 2001); https://doi.org/10.1117/12.434736

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