Nanoparticle-amplified surface plasmon resonance for detection of DNA hybridization

Glenn P. Goodrich; Sheila R. Nicewarner; Lin He; Michael J. Natan; Christine D. Keating

doi:10.1117/12.430764

18 June 2001 Nanoparticle-amplified surface plasmon resonance for detection of DNA hybridization

Glenn P. Goodrich, Sheila R. Nicewarner, Lin He, Michael J. Natan, Christine D. Keating

Proceedings Volume 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications; (2001) https://doi.org/10.1117/12.430764
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

In recent years there has been a great deal of interest in the measurement of DNA hybridization at surfaces. Surface-confined DNA hybridization has been used to monitor gene expression, to detect the presence of a particular DNA sequence and determine single nucleotide polymorphisms (SNPs). DNA microarrays, which can contain thousands of discrete DNA sequences on a single surface, have become widely used for hybridization studies. While a powerful technique, this technology is limited by the stability of the fluorescent dyes used to label the DNA, and the need to perform measurements ex-situ to reduce the fluorescence background. In this report, we describe the use of colloid-amplified surface plasmon resonance (SPR) to measure DNA hybridization at surfaces. SPR is a surface sensitive technique, which can be used to study hybridization in situ, and the use of colloidal metal tags provides excellent sensitivity. Angle-scanning SPR has been used to study oligonucleotide hybridization to surface confined probes, and work is underway to apply SPR imaging to study DNA hybridization in macro- and microarray formats.

Citation Download Citation

Glenn P. Goodrich, Sheila R. Nicewarner, Lin He, Michael J. Natan, and Christine D. Keating "Nanoparticle-amplified surface plasmon resonance for detection of DNA hybridization", Proc. SPIE 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications, (18 June 2001); https://doi.org/10.1117/12.430764

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