Nanocarriers of fluorescent probes and enzymes

Qiang Wang; Xiaoyan Chen; Dan Meisel; Hiroshi Mizukami; Agnes E. Ostafin

doi:10.1117/12.430767

18 June 2001 Nanocarriers of fluorescent probes and enzymes

Qiang Wang, Xiaoyan Chen, Dan Meisel, Hiroshi Mizukami, Agnes E. Ostafin

Proceedings Volume 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications; (2001) https://doi.org/10.1117/12.430767
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Cascade Blue, Sulforhodamine G and yeast alcohol dehydrogenase were encased inside nano-sized silicate shells and their absorption and fluorescence spectrophotometric properties, and the enzyme activity investigated. The stabilized molecules have potential in biosensors, drug delivery, and as recyclable catalysts. Cascade Blue and Sulforhodamine G were attached to 85 nm diameter colloidal gold, encased with silicate, and the gold core dissolved. Fluorescence quenched by the gold was recovered for both dyes, but the peak emission was red-shifted from that in water for Cascade Blue and blue-shifted for Sulforhodamine G. The excitation spectra of these dyes showed similar shifts, presumably reflecting their interaction with the shell interior. The spectrofluorometric results for alcohol dehydrogenase bound to 15 nm diameter colloidal gold were similar. The substrate ethanol and cofactor NAD were permeable to the silicate shell. Only 20% of enzyme activity of ADH was lost after binding to gold, and additional 20% lost by encasing with silicate. Subsequent rate of loss of activity was significantly lowered. This study demonstrated dyes and enzymes can be encased within silicate shells. Whether the shell protects these molecules from the environment, and how the thickness of silicate shells affects the rate of enzyme reactions remains to be investigated.

Citation Download Citation

Qiang Wang, Xiaoyan Chen, Dan Meisel, Hiroshi Mizukami, and Agnes E. Ostafin "Nanocarriers of fluorescent probes and enzymes", Proc. SPIE 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications, (18 June 2001); https://doi.org/10.1117/12.430767

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