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Proceedings Article

Confocal microscopy using a InGaN laser diode at 406 nm

[+] Author Affiliations
John M. Girkin, Allister I. Ferguson, David L. Wokosin, A. M. Gurney

Univ. of Strathclyde (United Kingdom)

Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, 30 (May 10, 2001); doi:10.1117/12.426782
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From Conference Volume 4260

  • Optical Diagnostics of Living Cells IV
  • Daniel L. Farkas; Robert C. Leif
  • San Jose, CA | January 20, 2001

abstract

We report on the application of a novel blue laser diode source to confocal microscopy. The source has the potential to be a replacement for argon lasers in a range of fluorescence based imaging systems. It has been demonstrated that with the use of a minimal number of optical components, high quality confocal images can be obtained from laser diodes operating around 406nm. Improvements in image quality through the use of anamorphic prisms to modify the beam profile have been investigated. Living mammalian cells stained with a range of biologically significant compounds have been imaged with high resolution. The stains excited range from fluorescein based compounds to green fluorescent protein. Through the use of the absorption wings a wide range of shorter wavelength fluorophores have been excites, including those more normally excited using UV laser systems. It is expected that this will lead to reduced photo-toxicity within the sample and conventional rather than UV transmitting objective lenses can be used.

© (2001) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

John M. Girkin ; Allister I. Ferguson ; David L. Wokosin and A. M. Gurney
"Confocal microscopy using a InGaN laser diode at 406 nm", Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, 30 (May 10, 2001); doi:10.1117/12.426782; http://dx.doi.org/10.1117/12.426782


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