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Proceedings Article

Infuence of fiber terminal face reflection on fiber optical confocal scanning microscope

[+] Author Affiliations
Lisong Yang

Shanghai Institute of Optics and Fine Mechanics (Ireland)

Guiying Wang, Jiangang Wang, Zhizhan Xu

Shanghai Institute of Optics and Fine Mechanics (China)

Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, 332 (September 17, 1999); doi:10.1117/12.364408
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From Conference Volume 3863

  • 1999 International Conference on Biomedical Optics
  • Qingming Luo; Britton Chance; Lihong V. Wang; Steven L. Jacques
  • Wuhan, China | October 25, 1999

abstract

In fiber optical confocal scanning imaging system of reflection mode, interference of the reflection light beams from terminal face of the fiber coupling tip and from the surface of the sample will always destroy the image of low reflection index samples, such as CD-R pregroove basal disc and biotic samples. A quantitative analysis was given to find out the influence of fiber terminal face reflection on the system axial response. In order to avoid the influence of the fiber reflected light, the reflectivity ratio of the fiber tip to the sample should be low. Interference noise was effectively reduced by immersing the unused fiber tip into glycerol and cleaving the fiber tip end face at an angle. The proof experimental results of axial response have been shown. Finally, good quality confocal images of the recordable CD pregroove and the chromosome were presented.

© (1999) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Lisong Yang ; Guiying Wang ; Jiangang Wang and Zhizhan Xu
"Infuence of fiber terminal face reflection on fiber optical confocal scanning microscope", Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, 332 (September 17, 1999); doi:10.1117/12.364408; http://dx.doi.org/10.1117/12.364408


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