0

Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

High-numerical-aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy

[+] Author Affiliations
Yoshihiro Kawano

Olympus America, Inc. (Japan)

Chikara Abe

Olympus America, Inc. (USA)

Teruo Kaneda, Yasushi Aono, Katsuyuki Abe, Keisuke Tamura

Olympus Optical Co., Ltd. (Japan)

Susumu Terakawa

Hamamatsu Univ. School of Medicine (Japan)

Proc. SPIE 4098, Optical Devices and Diagnostics in Materials Science, 142 (September 29, 2000); doi:10.1117/12.401623
Text Size: A A A
From Conference Volume 4098

  • Optical Devices and Diagnostics in Materials Science
  • David L. Andrews; Toshimitsu Asakura; Suganda Jutamulia; Wiley P. Kirk; Max G. Lagally; Ravindra B. Lal; James D. Trolinger
  • San Diego, CA | July 30, 2000

abstract

We designed Apo 100X NA 1.65 and Plan Apo 60X NA 1.45 objective lenses. When we use three lenses for water- immersed specimens, the marginal angle available for the total internal reflection becomes larger compared with NA 1.40 objective lens. Therefore, the alignment of the laser beam to the objective lens becomes very easy. We also designed the TIRFM illuminator. A single mode fiber is connected between the laser and illuminator. The laser beam is conducted through the fiber and supplied to the illuminator with a pre-adjusted positioning. Almost no additional alignment of illumination light is necessary.

© (2000) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Yoshihiro Kawano ; Chikara Abe ; Teruo Kaneda ; Yasushi Aono ; Katsuyuki Abe, et al.
"High-numerical-aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy", Proc. SPIE 4098, Optical Devices and Diagnostics in Materials Science, 142 (September 29, 2000); doi:10.1117/12.401623; http://dx.doi.org/10.1117/12.401623


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
 

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

Buy this article ($18 for members, $25 for non-members).
Sign In