Comparison of PDT efficiencies for photo-oxidation of substrate (NADPH) using a photosensitizer (Photofrin II)

Paulo R. Bargo; Parmeswararn Diagaradjane; Steven L. Jacques

doi:10.1117/12.379887

29 March 2000 Comparison of PDT efficiencies for photo-oxidation of substrate (NADPH) using a photosensitizer (Photofrin II)

Paulo R. Bargo, Parmeswararn Diagaradjane, Steven L. Jacques

Author Affiliations +

Proceedings Volume 3909, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX; (2000) https://doi.org/10.1117/12.379887
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Photo-oxidation was studied during PDT in a model system using NADPH as the target substrate and Photofrin II as the photosensitizer. The efficiency of NADPH oxidation per photon absorbed by photosensitizer was determined as a function of substrate concentration. The kinetics of changes in absorbance was measured to assay oxidation of NADPH. The efficiency of oxidation increased with increasing NADPH concentration and approached a stable value of 0.0048. Using typical values for the quantum efficiency of the Photofrin triplet state generation and the efficiency of singlet oxygen production we obtained a value of the efficiency of interaction between singlet oxygen and NADPH. Efficiency of diffusion of singlet oxygen was modeled with molecular diffusion theory using typical values for the singlet oxygen lifetime and the diffusion coefficient in aqueous solutions.

Citation Download Citation

Paulo R. Bargo, Parmeswararn Diagaradjane, and Steven L. Jacques "Comparison of PDT efficiencies for photo-oxidation of substrate (NADPH) using a photosensitizer (Photofrin II)", Proc. SPIE 3909, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX, (29 March 2000); https://doi.org/10.1117/12.379887

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