Assessment of ex-vivo and in-vivo near-infrared Raman spectroscopy for the classification of dysplasia within Barrett's esophagus

Martin G. Shim; Louis-Michel Wong Kee Song M.D.; Norman E. Marcon M.D.; Shirley Hassaram; Brian C. Wilson

doi:10.1117/12.384963

8 May 2000 Assessment of ex-vivo and in-vivo near-infrared Raman spectroscopy for the classification of dysplasia within Barrett's esophagus

Martin G. Shim, Louis-Michel Wong Kee Song M.D., Norman E. Marcon M.D., Shirley Hassaram, Brian C. Wilson

Author Affiliations +

Proceedings Volume 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques; (2000) https://doi.org/10.1117/12.384963
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The purpose of this study is to assess if Raman spectroscopy can classify dysplastic (DYS) and early neoplastic lesions within Barrett's esophagus (BE). In BE, the normal squamous epithelium (SQ) lining the esophagus is replaced by columnar epithelium. These patients have a 30-125 fold excess risk of developing adenocarcinoma. Raman spectroscopy may provide diagnostic information so that tissue transformation may be detected at an early stage and improve the patient's prognosis. Ex vivo measurements were carried out initially on biopsy samples obtained from BE patients undergoing routine endoscopic and biopsy surveillance. Differences were noted in the spectral regions 1200-1350 cm^-1 and 1550-1640 cm^-1 when comparing different histopathologic grades. Principal component analysis of the spectra led to good separation between SE and BE but not between BE and DYS. Improved results were obtained using a probabilistic artificial neural network, with a resultant sensitivity and specificity of 77 percent and 93 percent in differentiating SQ/BE from dysplasia, respectively. Recently, in vivo endoscopic measurements have been performed. These preliminary results indicate that RS in combination with endoscopy may be a useful technique to screen BE patients for dysplastic/early neoplastic lesions.

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Martin G. Shim, Louis-Michel Wong Kee Song M.D., Norman E. Marcon M.D., Shirley Hassaram, and Brian C. Wilson "Assessment of ex-vivo and in-vivo near-infrared Raman spectroscopy for the classification of dysplasia within Barrett's esophagus", Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); https://doi.org/10.1117/12.384963

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