Confocal imaging through scattering media with a volume holographic filter

Michal Balberg; George Barbastathis; Sergio Fantini; David J. Brady

doi:10.1117/12.384180

2 May 2000 Confocal imaging through scattering media with a volume holographic filter

Michal Balberg, George Barbastathis, Sergio Fantini, David J. Brady

Proceedings Volume 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII; (2000) https://doi.org/10.1117/12.384180
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The use of a volume holographic filter as collector element in a confocal system imaging through scattering (turbid) material is described. We show that the penetration depth of the volume holographic system is de-coupled from the scatter noise discrimination properties, and is potentially more advantageous than the traditional confocal microscope. Since the volume-holographic filter is a matched-filter, the penetration depth is dependent on the mismatch of the refractive index of the sample being imaged relative to the recording conditions. We present a method to overcome this limitation based on using a pre-compensating index matching film during the recording of the volume hologram. An improvement of the penetration depth is shown experimentally.

Citation Download Citation

Michal Balberg, George Barbastathis, Sergio Fantini, and David J. Brady "Confocal imaging through scattering media with a volume holographic filter", Proc. SPIE 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII, (2 May 2000); https://doi.org/10.1117/12.384180

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