Subsingle-molecule determination of nonfluorescent species by scanning thermal lens microscope and its application to single-cell measurement

Takehiko Kitamori; Marika Uchida; Akiko Egami; Kazuya Sekiguchi; Jinjian Zheng; Tsuguo Sawada; Manabu Tokeshi; Kiichi Sato; Hiroko Kimura

doi:10.1117/12.383356

21 April 2000 Subsingle-molecule determination of nonfluorescent species by scanning thermal lens microscope and its application to single-cell measurement

Takehiko Kitamori, Marika Uchida, Akiko Egami, Kazuya Sekiguchi, Jinjian Zheng, Tsuguo Sawada, Manabu Tokeshi, Kiichi Sato, Hiroko Kimura

Author Affiliations +

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383356
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We have developed a thermal lens microscope which indices and detects photothermal effect at sub-single molecules level in/on liquids and condensed media. The thermal lens microscope can determine non-fluorescent molecules without receiving serious effects of light scattering in/on various condensed phase substances, and it can be applied to imaging of the distribution of non-fluorescent molecules by scanning on the sample. These characteristics of the thermal lens microscope are suitable for ultra sensitive analysis and imaging of biomedical substance in/on a single cell, separation media, and microfabricated chemical devices. We applied the thermal lens microscope to determine an ultratrace chemical species in various media.

Citation Download Citation

Takehiko Kitamori, Marika Uchida, Akiko Egami, Kazuya Sekiguchi, Jinjian Zheng, Tsuguo Sawada, Manabu Tokeshi, Kiichi Sato, and Hiroko Kimura "Subsingle-molecule determination of nonfluorescent species by scanning thermal lens microscope and its application to single-cell measurement", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383356

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