One-step conjugation of biomolecules to luminescent nanocrystals

Warren C. W. Chan; Tara L. Prendergast; Manas Jain; Shuming Nie

doi:10.1117/12.384243

26 April 2000 One-step conjugation of biomolecules to luminescent nanocrystals

Warren C. W. Chan, Tara L. Prendergast, Manas Jain, Shuming Nie

Proceedings Volume 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation; (2000) https://doi.org/10.1117/12.384243
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The development of optical pulses is crucial to the elucidation of complex biological and chemical problems. Existing fluorescent labels tend to photobleach, have low luminescence, and area sensitive to the environment. These problems can be overcome by the use of semiconductor quantum dots. These ZnS-capped CdSe quantum dots are synthesized in an organic media. To use them for biological labeling, the quantum dots are made water-soluble by adsorbing bifunctional molecules onto the surface. This step is crucial for the successful conjugation of biomolecules onto the surface. In this report, a new method for the direct adsorption of biomolecules onto the surface of quantum dots is demonstrated. Biomolecules such as glutathione, mercaptusuccic acid, and histidine are directly conjugated to luminescent quantum dots by S-Zn or N-Zn bonding.

Citation Download Citation

Warren C. W. Chan, Tara L. Prendergast, Manas Jain, and Shuming Nie "One-step conjugation of biomolecules to luminescent nanocrystals", Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); https://doi.org/10.1117/12.384243

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