Investigation into surfactant-aided matrix-assisted laser desorption/ionization-mass spectrometry for peptide mapping

Rama Tummala; Lisa M. Ballard; Gary A. Breaux; Kari B. Green-Church; Patrick A. Limbach

doi:10.1117/12.380494

22 March 2000 Investigation into surfactant-aided matrix-assisted laser desorption/ionization-mass spectrometry for peptide mapping

Rama Tummala, Lisa M. Ballard, Gary A. Breaux, Kari B. Green-Church, Patrick A. Limbach

Author Affiliations +

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380494
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is a popular analytical tool for the analysis of large biomolecules such as proteins, oligonucleotides and oligosaccharides. This method has proven to be extremely useful for the analysis of water soluble proteins. However, analysis of hydrophobic proteins using MALDI-MS has been more of a challenge. The difficulty arises due to the limited solubility of these compounds in the aqueous solutions used to prepare the MALDI matrix. This study has investigated the effect of surfactants on the MALDI-MS data obtained from hydrophobic peptides and tryptic digests of hydrophobic and hydrophilic proteins. The surfactant sodium dodecylsulfate was found to improve the information content obtained during MALDI-MS analysis of such molecules. Preliminary results which examine the applicability of surfactant-aided MALDI-TOFMS for the characterization of proteolytic digests of proteins will be presented, and the potential use of this analytical strategy in a proteomics-based examination of intrinsic membrane proteins will be discussed.

Citation Download Citation

Rama Tummala, Lisa M. Ballard, Gary A. Breaux, Kari B. Green-Church, and Patrick A. Limbach "Investigation into surfactant-aided matrix-assisted laser desorption/ionization-mass spectrometry for peptide mapping", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380494

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available