Development of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments

Karen Y. Kavanagh; James Edward Walsh; J. Murphy; M. Harmey; M. A. Farrell; O. Hardimann; R. Perryman

doi:10.1117/12.276138

30 May 1997 Development of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments

Karen Y. Kavanagh, James Edward Walsh, J. Murphy, M. Harmey, M. A. Farrell, O. Hardimann, R. Perryman

Proceedings Volume 3105, Chemical, Biochemical and Environmental Fiber Sensors IX; (1997) https://doi.org/10.1117/12.276138
Event: Environmental Sensing III, 1997, Munich, Germany

Abstract

The continuing demand for non-invasive tools for use in clinical diagnosis has created the need for flexible and innovative optical systems which satisfy current requirements. We report the development of a microspectrophotometer system for use on mitochondrial respiratory pigments. This novel optical fiber set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Preliminary data is presented for the reduction of cytochrome-c by two methods. In the first, cytochrome-c was reduced in isolation using sodium dithionite. The second was an in-vivo simulation of the reduction of cytochrome-c using the mitochondrial extract from rat liver. The key features of the system are; front end adaptability, high sensitivity and fast scanning capabilities which are essential for the rapid biological reactions which are observed.

Citation Download Citation

Karen Y. Kavanagh, James Edward Walsh, J. Murphy, M. Harmey, M. A. Farrell, O. Hardimann, and R. Perryman "Development of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments", Proc. SPIE 3105, Chemical, Biochemical and Environmental Fiber Sensors IX, (30 May 1997); https://doi.org/10.1117/12.276138

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