Steady-state and time-resolved fluorometry of fluorescent pollutants and heavy metal complexes

Ute Resch; Knut Rurack

doi:10.1117/12.276139

30 May 1997 Steady-state and time-resolved fluorometry of fluorescent pollutants and heavy metal complexes

Ute Resch, Knut Rurack

Proceedings Volume 3105, Chemical, Biochemical and Environmental Fiber Sensors IX; (1997) https://doi.org/10.1117/12.276139
Event: Environmental Sensing III, 1997, Munich, Germany

Abstract

Time-resolved laser-induced fluorescence spectroscopy is one of the most sensitive optical methods which is well suited for on-line in situ analysis. Here, three examples for the steady- state and time-resolved fluorescence analysis of environmentally important analytes, the fluorescent monoaromatic hydrocarbons benzene, toluene, and xylene as well as non fluorescent heavy metal ions forming a fluorescent complex with a cation coordinating fluorescence probe, are presented and the potential of both methods is discussed. For BTX, various mixtures of the spectrally similar compounds B, T, and X showing different fluorescence lifetimes were studied with both methods. As an example for fluorometric metal ion analysis, the fluorescence probe BP(OH)₂ (2,2'-bipyridyl- 3,3'-diol) was employed for the determination of d¹⁰ metal ions in water and the newly developed fluorescence probe APTA for the detection of Cu(II). Cation complexation of BP(OH₂ yields spectrally very similar complexes which differ in their fluorescence lifetimes. Complexation of APTA to Cu(II) leads to small spectral changes and a strong increase in fluorescence quantum yield and lifetime. For the analytes studied, a comparison of the detection limits, standard deviations, and linear dynamic range of both methods clearly demonstrates the analytical potential of time-resolved fluorometry.

Citation Download Citation

Ute Resch and Knut Rurack "Steady-state and time-resolved fluorometry of fluorescent pollutants and heavy metal complexes", Proc. SPIE 3105, Chemical, Biochemical and Environmental Fiber Sensors IX, (30 May 1997); https://doi.org/10.1117/12.276139

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