Prediction of ALA-PDT efficacy through remote color inspection and post-therapy sequential histologic observations of skin malignancies

Andronicki D. Tosca; Constantin J. Balas; Maria P. Stefanidou; John C. Katsantonis; Savas K. Georgiou; Maria N. Tzardi; Emmanuel S. Helidonis M.D.

doi:10.1117/12.297807

29 December 1997 Prediction of ALA-PDT efficacy through remote color inspection and post-therapy sequential histologic observations of skin malignancies

Andronicki D. Tosca, Constantin J. Balas, Maria P. Stefanidou, John C. Katsantonis, Savas K. Georgiou, Maria N. Tzardi, Emmanuel S. Helidonis M.D.

Author Affiliations +

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297807
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Twenty nine skin malignancies, 14 solar keratoses (SK) and 15 basal cell carcinomas (BCC) were treated with ALA-PDT. The overall cure response rate for SK was 85.7% and 66.6% for BCC. Specific histologic findings in sequential histology were indicative of the destructive and restoration mechanisms. A multispectral imaging system (MUSIS) was developed to perform diffuse reflection and fluorescence studies. Most of the lesions developed peak fluorescence 1.5 - 4 hours after ALA application. Hue was the most sensitive parameter to record the kinetics of the photosensitizer concentration in the atypical cells. It was worth noticing that the peak fluorescence differed among patients and this was an indication to start the irradiation more objectively. Erythema inspection and quantification during irradiation and afterwards was achieved by means of MUSIS. Erythema development was shown to include a new peak in the Hue histogram corresponding to a color range between red and violet. Time course erythema elicitation was found to differ between SK and BCC in accordance with recorded histologic difference relative to PDT-destruction mechanisms. Erythema development was shown to correlate significantly with the phototoxic effect and might be considered a reliable predictor of PDT efficacy.

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Andronicki D. Tosca, Constantin J. Balas, Maria P. Stefanidou, John C. Katsantonis, Savas K. Georgiou, Maria N. Tzardi, and Emmanuel S. Helidonis M.D. "Prediction of ALA-PDT efficacy through remote color inspection and post-therapy sequential histologic observations of skin malignancies", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297807

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