Colon cancer diagnosis using fluorescence spectroscopy and fluorescence imaging technique

Dido M. Yova; Vassilis Atlamazoglou; P. Davaris; Nikolaos Kavantzas; Spyros Loukas

doi:10.1117/12.297946

29 December 1997 Colon cancer diagnosis using fluorescence spectroscopy and fluorescence imaging technique

Dido M. Yova, Vassilis Atlamazoglou, P. Davaris, Nikolaos Kavantzas, Spyros Loukas

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297946
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

It is well known that fluorescence spectroscopy can provide information about the differences in the concentration of chromophores in healthy and cancerous tissues. The tumor detection potential can be enhanced by using exogenous fluorescent agents with selective accumulation in cancerous tissue. In this study healthy and cancerous human colon tissue samples were obtained after colon surgery. Excitation-emission matrices were collected using a fluorescence spectrometer. The optimum excitation wavelength lied at 340 nm. After the acquisition of autofluorescence spectra, the samples were incubated in a solution of 4 (mu) g/ml of Rhodamine analogs. Rhodamine B, Rhodamine 6G and three recently synthesized analogs, were used. For the acquisition of fluorescence images, an endoscopic imaging system was developed. Fluorescence imaging with the concomitant use of Rhodamine analogs revealed a remarkable differentiation of cancerous from healthy colonic mucosa.

Citation Download Citation

Dido M. Yova, Vassilis Atlamazoglou, P. Davaris, Nikolaos Kavantzas, and Spyros Loukas "Colon cancer diagnosis using fluorescence spectroscopy and fluorescence imaging technique", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297946

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available