Autofluorescence of basal cell carcinoma

Haishan Zeng; David I. McLean M.D.; Calum E. MacAulay; Branko Palcic; Harvey Lui M.D.

doi:10.1117/12.312300

1 July 1998 Autofluorescence of basal cell carcinoma

Haishan Zeng, David I. McLean M.D., Calum E. MacAulay, Branko Palcic, Harvey Lui M.D.

Proceedings Volume 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII; (1998) https://doi.org/10.1117/12.312300
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

5To assess the potential of optical spectroscopy and imaging for improving the detection and evaluation of early skin cancer, we have been studying autofluorescence properties of various skin diseases. In this paper, the autofluorescence properties of basal cell carcinoma (BCC) under 442 nm excitation will be presented. Three different spectroscopy and imaging tools have been used in this study: (1) a portable fiber optic spectrometer for in vivo spectral measurements in an outpatient clinic; (2) a CCD imaging system for cutaneous autofluorescence imaging in vivo; and (3) a fiber optic microspectrophotometer system for frozen tissue section examination of in vitro microscopic fluorophore distribution and spectroscopy. Autofluorescence spectra from 109 BCC lesions characteristically showed decreased fluorescence intensity for BCC as compared to the surrounding normal skin. Of 109 biopsy-proven BCC lesions, 104 showed a decreased fluorescence signal. Decreased autofluorescence in BCC has also been corroborated by in vivo autofluorescence imaging of BCC lesions. Microscopic data from histologic frozen sections mirror the in vivo measurements, with decreased autofluorescence observed within and immediately around basal cell tumor nests. An optical model of basal cell carcinoma is under construction.

Citation Download Citation

Haishan Zeng, David I. McLean M.D., Calum E. MacAulay, Branko Palcic, and Harvey Lui M.D. "Autofluorescence of basal cell carcinoma", Proc. SPIE 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII, (1 July 1998); https://doi.org/10.1117/12.312300

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