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Proceedings Article

Compact LED-based phase fluorimeter detection system for chemical and biosensor arrays

[+] Author Affiliations
Emmanuil M. Rabinovich, Michael J. O'Brien II, Balaji Srinivasan, Steve Elliott, Xiang-Cun Long, Ravinder K. Jain, Victor H. Perez-Luna, Yuqing Zhou, Leonard M. Tender, Gabriel P. Lopez

Univ. of New Mexico (USA)

Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, 2 (March 26, 1998); doi:10.1117/12.304372
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From Conference Volume 3258

  • Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications
  • Paul L. Gourley
  • San Jose, CA | January 27, 1998

abstract

This paper describes a design for an inexpensive phase fluorimeter based on blue or green light emitting diodes for use with chemical and biological sensors with fluorescence and phosphorescence lifetime-based transduction. The phase fluorimeter is based on a personal computer, two frequency synthesizers and off-the-shelf optical and electronic components and has an estimated lifetime resolution better than 10 ps. The phase fluorimeter is especially well-suited for implementation with arrays of chemically sensitive elements. The data acquisition system allows rapid monitoring of light emission from fluors or phosphors immobilized in the chemically sensitive array elements, each of which can be designed to be responsive to a particular chemical analyte. This paper describes chemically sensitive elements based on ultrathin films of porous polymers and on self-assembled monolayers. The paper focuses on methods for detection of low levels of luminescence emission from micropatterned array elements that comprise sensor arrays. Of particular importance is the detection of low levels of fluorescence and phosphorescence from SAMs of alkanethiolates on thin gold and silver films. Methods for enhancing the luminescence yield from these SAMs include optimization of the dielectric environment of the luminescent dyes and surface plasmon resonance enhanced excitation.

© (1998) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Emmanuil M. Rabinovich ; Michael J. O'Brien II ; Balaji Srinivasan ; Steve Elliott ; Xiang-Cun Long, et al.
"Compact LED-based phase fluorimeter detection system for chemical and biosensor arrays", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, 2 (March 26, 1998); doi:10.1117/12.304372; http://dx.doi.org/10.1117/12.304372


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