Quantitative imaging of assays in miniaturized plate formats

Gang Sun; Peter Ramm

doi:10.1117/12.307331

10 April 1998 Quantitative imaging of assays in miniaturized plate formats

Gang Sun, Peter Ramm

Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307331
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Miniaturized sample formats offer advantages in cost and throughput to screening laboratories. Imaging systems may be valuable tolls for assay miniaturization. We describe fluorescence performance of our Tundra^TM assay imaging system, which incorporates a thinned, back illuminated CCD area detector, a telecentric lens with internal excitation optics, and AssayVision^TM software. Using a flat field correction, Tundra imaged a difficult (faint) gel with performance similar to that of a high performance scanning laser. The accuracy of Tundra's dedicated well plate calibration functions was also evaluated in 384 (40 (mu) l sample volume) and 1536 (5 (mu) l) well plates, and across a realistic range of fluor concentrations. With both plate formats, and across the full range of fluor concentrations, Tundra shows coefficients of variation which are similar to those of a scanning laser, and to those reported for PMT devices. Tundra also exhibits highly linear response to fluor concentration.

Citation Download Citation

Gang Sun and Peter Ramm "Quantitative imaging of assays in miniaturized plate formats", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307331

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KEYWORDS

Calibration

Imaging systems

Luminescence

Sensors

Cameras

Optical filters

Back illuminated sensors

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