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Proceedings Article

Three-dimensional limited-angle microtomography of blood cells: experimental results

[+] Author Affiliations
Gennady G. Levin, Gennady N. Vishnyakov, Constantin S. Zakarian

Russian Research Institute for Optophysical Measurements (Russia)

Alexey V. Likhachov, Valery V. Pickalov

Institute of Theoretical and Applied Mechanics (Russia)

Gennady I. Kozinets, Julia K. Novoderzhkina, Elena A. Streletskaya

Hematology Scientific Ctr./Russian Academy of Medical Science (Russia)

Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, 159 (June 9, 1998); doi:10.1117/12.310549
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From Conference Volume 3261

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V
  • Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Thomas T. Lu; Tony Wilson
  • San Jose, CA | January 27, 1998

abstract

Tomographic measurements of the 3D refractive index spatial distribution within optically transparent phase samples with computerized interferometric microscopes are proposed. Phase shifting interferometric microtomography applications for the 3D image reconstruction of the blood cells are represented. The immersion 100x, N.A. equals 1.25 objective was used to increase the spatial resolution and observation angle range to 90 degree. ART, combined ART and iterative Gerchberg-Papoulis 3D algorithm were used for the tomogram reconstruction. To determine the accuracy and spatial resolution of the blood cells image reconstruction by means of the interferometric microtomographic method the numerical simulations were implemented.

© (1998) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Gennady G. Levin ; Gennady N. Vishnyakov ; Constantin S. Zakarian ; Alexey V. Likhachov ; Valery V. Pickalov, et al.
"Three-dimensional limited-angle microtomography of blood cells: experimental results", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, 159 (June 9, 1998); doi:10.1117/12.310549; http://dx.doi.org/10.1117/12.310549


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