Tracking on treelike structures in 3D confocal images

Andreas Herzog; Gerald Krell; Bernd Michaelis; Werner Zuschratter

doi:10.1117/12.310550

9 June 1998 Tracking on treelike structures in 3D confocal images

Andreas Herzog, Gerald Krell, Bernd Michaelis, Werner Zuschratter

Proceedings Volume 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V; (1998) https://doi.org/10.1117/12.310550
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Confocal microscopy is qualified to perform volume scans of nerve cells with dendrites and spines. Length and diameter of the dendrite branches and the spines should be determined to analyze the influence of learning processes. A prerequisite for that is the recognition of the dendritic structure with branching off and spines. Because the microscope operates at the resolution limit the images are blurry, noisy and only poorly sampled. In contrast to other methods which are based on binary images and thinning algorithms, our method tracks and dendritic tree faster and in the gray-level domain using simple geometric models.An explicit segmentation is unnecessary and knowledge about shape and structure of the dendrite is included as a priori information. For large trees, first a low resolution scan is captured to crete a rough model. The algorithm allows to refine this model using higher resolution scans for interesting regions along the dendrite. The large unimportant areas between the dendrite branches are not scanned at high resolution to save time and disc space. In a second step, the parameters of the model are adapted to the microscope image by minim zing the deviation of the microscope image from the mode image convolved by the microscope point spread function. Features like number, diameter, length and position of the dendrite branches and spines can be easily calculated from the model. An interactive user intervention is possible at the model domain.

Citation Download Citation

Andreas Herzog, Gerald Krell, Bernd Michaelis, and Werner Zuschratter "Tracking on treelike structures in 3D confocal images", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); https://doi.org/10.1117/12.310550

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