Three-dimensional organization of chromosomes studied by in-situ hybridization and optical-sectioning microscopy

Yasushi Hiraoki; Mary C. Rykowski; Jeffrey A. Lefstin; David A. Agard; John W. Sedat

doi:10.1117/12.17779

1 August 1990 Three-dimensional organization of chromosomes studied by in-situ hybridization and optical-sectioning microscopy

Yasushi Hiraoki, Mary C. Rykowski, Jeffrey A. Lefstin, David A. Agard, John W. Sedat

Author Affiliations +

Proceedings Volume 1205, Bioimaging and Two-Dimensional Spectroscopy; (1990) https://doi.org/10.1117/12.17779
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

We have examined the three-dimensional arrangement of chromosomes in embryos of Drosophila melanogaster at the syncytial blastoderm stage using three-dimensional optical sectioning microscopy. High-resolution optical sectioning in fixed embryos, in conjunction with in situ hybridization, has revealed the location of specific chromosomal regions within diploid interphase nuclei, as well as the spatial arrangement of mitotic chromosomes. Time-lapse in vivo optical sectioning has revealed the dynamic behavior of chromosomes throughout the mitotic cycle. Combination of these observations has provided insights into the dynamic aspects of three-dimensional chromosome behavior.

Citation Download Citation

Yasushi Hiraoki, Mary C. Rykowski, Jeffrey A. Lefstin, David A. Agard, and John W. Sedat "Three-dimensional organization of chromosomes studied by in-situ hybridization and optical-sectioning microscopy", Proc. SPIE 1205, Bioimaging and Two-Dimensional Spectroscopy, (1 August 1990); https://doi.org/10.1117/12.17779

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