Charge-coupled devices for quantitative confocal microscopy of the eye

Barry R. Masters; Tim Bruchman; Guoqing Xiao; Gordon S. Kino

doi:10.1117/12.19435

1 July 1990 Charge-coupled devices for quantitative confocal microscopy of the eye

Barry R. Masters, Tim Bruchman, Guoqing Xiao, Gordon S. Kino

Proceedings Volume 1242, Charge-Coupled Devices and Solid State Optical Sensors; (1990) https://doi.org/10.1117/12.19435
Event: Electronic Imaging: Advanced Devices and Systems, 1990, Santa Clara, CA, United States

Abstract

The use of charge-coupled devices (CCD) for quantitative microscopy has advantages over standard video detectors. For quantitative imaging in the life sciences the use of CCDs have the following advantages: it is a quantitative photodetector, with a large dynamic range and high quantum efficiency. The advantage of a large dynamic range of the order 16,000:1 (14-bits) is important in confoca]. microscopy where there are regions of extremely low light intensity and regions with high intensity. The linear factor of the dynamic range is important for quantitation of the images. Another important property for biological imaging is sensitivity. The use of back- illuminated, UV enhanced coatings, thinned CCD devices, with anti-reflection coatings all result in higher quantum efficiencies. Slow scan devices can be used in a special mode to capture an image in less than a video frame, however, they are most useful as linear integrating light detectors. In order to demonstrate some of the useful properties of CCD detectors, we have used a CCD detection system to image low light level signals from living transparent biological material. A CCD camera was coupled to a confoca]. microscope and images were collected in reflected light. The samples included the cornea, and the in situ ocular lens. The quality of the images is demonstrated over a wide range of light levels. The images of the eye at submicron resolution clearly demonstrate the advantages of using a solid state charge-coupled device detector for quantitative confocal reflected light microscopy of thick, transparent ocular tissue.

Citation Download Citation

Barry R. Masters, Tim Bruchman, Guoqing Xiao, and Gordon S. Kino "Charge-coupled devices for quantitative confocal microscopy of the eye", Proc. SPIE 1242, Charge-Coupled Devices and Solid State Optical Sensors, (1 July 1990); https://doi.org/10.1117/12.19435

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