Photodynamic tumor therapy and on-line fluorescence spectroscopy after aminolevulinic acid administration using 633-nm light as therapeutic and fluorescence excitation radiation

Karsten Koenig; Alwin Kienle; Wolf-Henning Boehncke; Roland Kaufmann; Angelika C. Rueck; Thomas H. Meier; Rudolf W. Steiner

doi:10.1117/12.168686

1 March 1994 Photodynamic tumor therapy and on-line fluorescence spectroscopy after aminolevulinic acid administration using 633-nm light as therapeutic and fluorescence excitation radiation

Karsten Koenig, Alwin Kienle, Wolf-Henning Boehncke, Roland Kaufmann, Angelika C. Rueck, Thomas H. Meier, Rudolf W. Steiner

Author Affiliations +

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168686
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

PDT and on-line fluorescence spectroscopy were carried out on human tumors after ALA- administration using 633 nm-light of a dye laser as therapeutic radiation and as fluorescence excitation radiation. This has the following advantages: (1) use of one laser for PDT and fluorescence diagnosis only, (2) the possibility of on-line fluorescence measurements, and (3) excitation of protoporphyrin molecules in deep tissue layers. Monte Carlo calculations were carried out to determine the excitation and fluorescence photon distribution in the case of red and violet excitation radiation. The results show the possibility of depth-resolved measurements on the fluorophore distribution by variation of the excitation wavelength. The influence of remitted excitation light and of the spontaneous radiation from the laser as well as the possible excitation of food-based degradation products of chlorophyll has to be considered in high-sensitive fluorescence measurements.

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Karsten Koenig, Alwin Kienle, Wolf-Henning Boehncke, Roland Kaufmann, Angelika C. Rueck, Thomas H. Meier, and Rudolf W. Steiner "Photodynamic tumor therapy and on-line fluorescence spectroscopy after aminolevulinic acid administration using 633-nm light as therapeutic and fluorescence excitation radiation", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168686

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