In-vivo absorption properties of algal pigments

Robert R. Bidigare; Michael E. Ondrusek; John H. Morrow; Dale A. Kiefer

doi:10.1117/12.21451

1 September 1990 In-vivo absorption properties of algal pigments

Robert R. Bidigare, Michael E. Ondrusek, John H. Morrow, Dale A. Kiefer

Proceedings Volume 1302, Ocean Optics X; (1990) https://doi.org/10.1117/12.21451
Event: 1990 Technical Symposium on Optics, Electro-Optics, and Sensors, 1990, Orlando, FL, United States

Abstract

Estimates of the in vivo specific absorption coefficients (m2 mg'; 400-750 nm, 2 nm intervals) for the major algal pigment groups (chlorophylls, carotenoids and phycobilins) are presented. "Unpackaged" absorption coefficients were initially obtained by measuring the absorption properties of pure pigment standards spectrophotometrically and "shifting" their absorption maxima to match in vivo positions. Two approaches for estimating the phytoplankton absorption coefficient (spectral reconstruction and spectral decomposition) are compared by linear regression analysis, incorporating concurrent measurements of particulate absorption and pigmentation performed in the Sargasso Sea. Results suggest that "pigment package" effects are minimal for natural assemblages of open-oceanic phytoplankton and that accessory pigments do not always co-vary with chlorophyll a over depth and time.

Citation Download Citation

Robert R. Bidigare, Michael E. Ondrusek, John H. Morrow, and Dale A. Kiefer "In-vivo absorption properties of algal pigments", Proc. SPIE 1302, Ocean Optics X, (1 September 1990); https://doi.org/10.1117/12.21451

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