Flow immunosensor detection of explosives and drugs of abuse

Anne W. Kusterbeck; Linda L. Judd; Hao Yu; June Myles; Frances S. Ligler

doi:10.1117/12.171242

28 March 1994 Flow immunosensor detection of explosives and drugs of abuse

Anne W. Kusterbeck, Linda L. Judd, Hao Yu, June Myles, Frances S. Ligler

Proceedings Volume 2092, Substance Detection Systems; (1994) https://doi.org/10.1117/12.171242
Event: Substance Identification Technologies, 1993, Innsbruck, Austria

Abstract

An antibody-based biosensor has been developed at the Naval Research Laboratory which is capable of detecting both drugs and explosives present at low levels in an aqueous sample. In the flow immunosensor, antibodies are immobilized onto a solid substrate, allowed to bind a fluorescently labeled signal molecule, placed in a small column and attached to a buffer flow. Upon sample introduction, an amount of the fluorescent signal molecule is released that is proportional to the concentration of applied sample. The response time of the sensor is under a minute, and multiple samples can be analyzed without the need for additional reagents. Quantitative assays are being developed for a variety of compounds, including TNT, DNT, PETN, and cocaine. The laboratory prototype has been used to study how choice of fluorophore, antibody density, and flow rate affect the signal intensity and column lifetime. A self-contained commercial instrument which can analyze up to seven different compounds from a single sample is currently being engineered under a Cooperative Research and Development Agreement.

Citation Download Citation

Anne W. Kusterbeck, Linda L. Judd, Hao Yu, June Myles, and Frances S. Ligler "Flow immunosensor detection of explosives and drugs of abuse", Proc. SPIE 2092, Substance Detection Systems, (28 March 1994); https://doi.org/10.1117/12.171242

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