Image processing and trapping of microscopic objects using a phase conjugate Michelson interferometer

Arthur E. T. Chiou; John H. Hong; Gregory J. Sonek; Yagang Liu; Michael W. Berns

doi:10.1117/12.180758

28 July 1994 Image processing and trapping of microscopic objects using a phase conjugate Michelson interferometer

Arthur E. T. Chiou, John H. Hong, Gregory J. Sonek, Yagang Liu, Michael W. Berns

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180758
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

We report the feasibility study of a versatile optical configuration consisting of a phase conjugate Michelson interferometer in conjunction with microscopic imaging optics for image processing and trapping of microscopic objects. Our test samples include phase gratings, amplitude gratings (i.e., Ronchi rulings), polystyrene microspheres, and biological samples such as liposomes and yeast cells. We have experimentally demonstrated (1) the novelty filtering feature which enhances the image of moving phase objects by suppressing the stationary background, (2) contrast reversal which is useful for the imaging of light absorbing (or scattering) particles, (3) the aberration correction capability of the system to enhance the image quality of microscopic objects embedded in or otherwise distorted by aberrators, and (4) optical trapping of polystyrene microspheres. The potential of using this technique for the manipulation and diagnosis of biological cells and tissues is discussed.

Citation Download Citation

Arthur E. T. Chiou, John H. Hong, Gregory J. Sonek, Yagang Liu, and Michael W. Berns "Image processing and trapping of microscopic objects using a phase conjugate Michelson interferometer", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180758

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