Aggregation and deformation of red blood cells as probed by a laser light scattering technique in a concentrated suspension: methodology

Amir H. Gandjbakhche; Pierre Mills; Patrick Snabre; Jacques Dufaux

doi:10.1117/12.180775

21 July 1994 Aggregation and deformation of red blood cells as probed by a laser light scattering technique in a concentrated suspension: methodology

Amir H. Gandjbakhche, Pierre Mills, Patrick Snabre, Jacques Dufaux

Author Affiliations +

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180775
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A light scattering technique is presented which investigates the backscattered and transmitted flux of He-Ne laser light illuminating a concentrated suspension of red blood cells (RBC) submitted to a simple shear flow. Our experiments show that the angular distributions of scattered light are closely related to the state of the suspension (at rest, or submitted to a simple shear flow) and the rheological parameters of the suspension such as viscosity and volume concentration. Transmitted and reflected light measurements demonstrated reproducible and predictable changes in scattering cross sections of oriented and deformed RBCs by shear flow. Additionally, it is shown that the scattering cross-section of aggregated RBCs is different from disaggregated RBCs. A theoretical model, substantiated by Monte- Carlo simulations, is proposed which relates the changes in shape of the angular distribution of scattered light to the rate of aggregation and the gradients of deformation of RBCs.

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Amir H. Gandjbakhche, Pierre Mills, Patrick Snabre, and Jacques Dufaux "Aggregation and deformation of red blood cells as probed by a laser light scattering technique in a concentrated suspension: methodology", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180775

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