Laser-induced saturation of fluorescence for complex organic molecules

Victor I. Yuzhakov; K. G. Yevsyukhina; Svetlana V. Patsayeva

doi:10.1117/12.308644

26 May 1998 Laser-induced saturation of fluorescence for complex organic molecules

Victor I. Yuzhakov, K. G. Yevsyukhina, Svetlana V. Patsayeva

Proceedings Volume 3404, ALT'97 International Conference on Laser Surface Processing; (1998) https://doi.org/10.1117/12.308644
Event: ALT '97 International Conference on Laser Surface Processing, 1997, Limoges, France

Abstract

The effect of fluorescence saturation is caused by limited life-time of molecules in excited state, and appears as a non-linear dependence of detected fluorescence signal versus excitation intensity under laser pulsed irradiation of the sample. It is noticeable at conditions of 10²⁴ exciting photons per second per square centimeter, which we usually have for laboratory laser fluorimeter. Spectroscopy of saturated fluorescence is a promising tool for studying such spectral molecular characteristics, as fluorescence life- time, absorption cross-section, constant of singlet-singlet annihilation, and their changes due to molecular interaction. The work presents the theory of the effect considering the cases of different intermolecular interaction (quenching mechanisms, singlet-singlet annihilation) and describes the experimental results on florescence saturation for several organic dyes in water.

Citation Download Citation

Victor I. Yuzhakov, K. G. Yevsyukhina, and Svetlana V. Patsayeva "Laser-induced saturation of fluorescence for complex organic molecules", Proc. SPIE 3404, ALT'97 International Conference on Laser Surface Processing, (26 May 1998); https://doi.org/10.1117/12.308644

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