Biotinylated polyalkylthiophene thin films and monolayers that specifically incorporate phycobiliproteins: toward smart materials

Madhu Sudhan Rao Ayyagari; Rajiv Pande; Jeong Ok Lim; Manohar Kamath; Nagendra Beladakere; Harry Hong Gao; Kenneth A. Marx; Sukant K. Tripathy; Jayant Kumar; Lynne A. Samuelson; Joseph A. Akkara; David L. Kaplan

doi:10.1117/12.174048

1 May 1994 Biotinylated polyalkylthiophene thin films and monolayers that specifically incorporate phycobiliproteins: toward smart materials

Madhu Sudhan Rao Ayyagari, Rajiv Pande, Jeong Ok Lim, Manohar Kamath, Nagendra Beladakere, Harry Hong Gao, Kenneth A. Marx, Sukant K. Tripathy, Jayant Kumar, Lynne A. Samuelson, Joseph A. Akkara, David L. Kaplan

Author Affiliations +

Proceedings Volume 2189, Smart Structures and Materials 1994: Smart Materials; (1994) https://doi.org/10.1117/12.174048
Event: 1994 North American Conference on Smart Structures and Materials, 1994, Orlando, FL, United States

Abstract

We are investigating thin film and monolayer systems that involve conjugated conducting polymers and specific biological macromolecules. One class of conducting polymers, polyalkylthiophenes, are derivatized with biotin. These biotinylated polymers form the basis for a generic cassette system of attachment for any biological molecule through biotinylation or interaction with streptavidin. The high affinity of the biotin-streptavidin system, used in sequential steps, forms the basis of the cassette method. We have formed both monolayers and thin films (a few nanometers) of the cassette assembly in which phycobiliproteins are incorporated. We are investigating the optical signal transduction properties of specific phycobiliproteins (phycoerythrin, phycocyanin and allophycocyanain) using the cassette system on the inner surface of glass capillaries and on optical fiber surfaces. Phycobiliprotein photocurrent signals in conducting polymer matrices on microelectrodes are also being investigated. Our aim is to integrate the signal transduction mechanisms of the phycobiliproteins within monolayers or thin films of the conducting polymers to create biosensors and related smart materials for applications in biomedicine and biotechnology.

Citation Download Citation

Madhu Sudhan Rao Ayyagari, Rajiv Pande, Jeong Ok Lim, Manohar Kamath, Nagendra Beladakere, Harry Hong Gao, Kenneth A. Marx, Sukant K. Tripathy, Jayant Kumar, Lynne A. Samuelson, Joseph A. Akkara, and David L. Kaplan "Biotinylated polyalkylthiophene thin films and monolayers that specifically incorporate phycobiliproteins: toward smart materials", Proc. SPIE 2189, Smart Structures and Materials 1994: Smart Materials, (1 May 1994); https://doi.org/10.1117/12.174048

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available