Integrated capillary electrophoresis using glass and plastic chips for multiplexed DNA analysis

Aran Paulus; Stephen J. Williams; Alexander P. Sassi; Pin H. Kao; Hongdong Tan; Herbert H. Hooper

doi:10.1117/12.322100

10 September 1998 Integrated capillary electrophoresis using glass and plastic chips for multiplexed DNA analysis

Aran Paulus, Stephen J. Williams, Alexander P. Sassi, Pin H. Kao, Hongdong Tan, Herbert H. Hooper

Proceedings Volume 3515, Microfluidic Devices and Systems; (1998) https://doi.org/10.1117/12.322100
Event: Micromachining and Microfabrication, 1998, Santa Clara, CA, United States

Abstract

Micromachined devices made of plastic have been used for fast electrophoretic separations using short separation distances and high electric field strengths. Unlike their glass counterparts, plastic chips can be manufactured economically and in high volume. Analysis can be performed in single channels, as shown for DNA sequencing mixtures, or in channel arrays as demonstrated for the analysis of ds DNA fragments. Compared to slab gel electrophoresis and capillary electrophoresis, separations are extremely fast with a time-scale under 20 minutes for a sequence analysis and under 2 minutes for fragment analysis. Confocal laser- induced fluorescence provides a sensitive means of detection.

Citation Download Citation

Aran Paulus, Stephen J. Williams, Alexander P. Sassi, Pin H. Kao, Hongdong Tan, and Herbert H. Hooper "Integrated capillary electrophoresis using glass and plastic chips for multiplexed DNA analysis", Proc. SPIE 3515, Microfluidic Devices and Systems, (10 September 1998); https://doi.org/10.1117/12.322100

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