Remote Raman spectroscopic imaging of human artery wall

Darith Phat; Phat Ngoc Vuong; Pascal Plaza; Francis Cheilan; Nguyen Quy Dao

doi:10.1117/12.48228

1 November 1991 Remote Raman spectroscopic imaging of human artery wall

Darith Phat, Phat Ngoc Vuong, Pascal Plaza, Francis Cheilan, Nguyen Quy Dao

Proceedings Volume 1525, Future Trends in Biomedical Applications of Lasers; (1991) https://doi.org/10.1117/12.48228
Event: MedTech '91, 1991, Berlin, Germany

Abstract

Fourier-transform (FT) Raman spectroscopy using near-infrared (IR) excitation has been studied as a mean to diagnose pathology in human tissues by differentiating between the six types of atherosclerotic and healthy tissues. The importance of this technique lies in the fact that with minimal surgical invasion of the Raman scattering can be collected remotely through optical fibers with a near-IR FT Raman spectrometer. To date, 17 fresh autopsic human-aorta samples were studied in vitro using Nd:YAG laser and a power varying from 50 to 80 mW. Raman spectroscopy indicated a broad-continuum emission between 100 and 3500 cm-1 with Raman peaks of almost equal intensity at wavelengths of 1450 and 1659 cm-1 (R equals ratio; 0.96 +/- 0.06) for healthy intima. Fatty streaks (type I lesion), uncalcified atheromas (type II: lipid, fibrous, and mixed), ulcered lesions (type IIIu) and ulcero-calcified lesions (type IIIu+c, all with low Raman intensity, were found to have lower Raman intensity peak ratio values of 0.74 +/- 0.040, 0.79 +/- 0.022, 0.88 +/- 0.017, and 0.83 +/- 0.016 (p c) were characterized by very strong overall Raman intensity, very low peak ratio (0.57 +/- 0.062, p -1 allowing for precise diagnosis. This diagnostic information, which was based on intensity ratios (I1659/I1450), correlated well with histologic and biochemical compositions. These findings were not only crucial for the achievement of successful laser angioplasty but also promising for the in vivo studies of atherogenesis.

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Darith Phat, Phat Ngoc Vuong, Pascal Plaza, Francis Cheilan, and Nguyen Quy Dao "Remote Raman spectroscopic imaging of human artery wall", Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); https://doi.org/10.1117/12.48228

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