Biological agent identification by nucleic acid base-pair analysis using surface-enhanced Raman spectroscopy

Stuart Farquharson; Wayne W. Smith; Susan Elliott; Jay F. Sperry

doi:10.1117/12.336858

18 January 1999 Biological agent identification by nucleic acid base-pair analysis using surface-enhanced Raman spectroscopy

Stuart Farquharson, Wayne W. Smith, Susan Elliott, Jay F. Sperry

Author Affiliations +

Proceedings Volume 3533, Air Monitoring and Detection of Chemical and Biological Agents; (1999) https://doi.org/10.1117/12.336858
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

Recently, a number of analytical methods have been successfully developed which use nucleic acid sequencing to identify biological warfare agents. However, the effectiveness of these methods, towards the safety and protection of US Armed Forces and their allies are limited by the period required to enumerate the nucleic acid through polymerase chain reactions or culture growth to produce sufficient quantities for analysis. To overcome this limitation, we have been investigating the ability of surface-enhanced Raman spectroscopy to detect nucleic acids with sufficient sensitivity and selectivity to eliminate the need for enumeration. The design of a small volume electrolytic sample cell will be presented along with analysis of the nucleic acid bases and preliminary analysis of model bacteria.

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Stuart Farquharson, Wayne W. Smith, Susan Elliott, and Jay F. Sperry "Biological agent identification by nucleic acid base-pair analysis using surface-enhanced Raman spectroscopy", Proc. SPIE 3533, Air Monitoring and Detection of Chemical and Biological Agents, (18 January 1999); https://doi.org/10.1117/12.336858

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