Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Comparative study of optical fluorescent nanosensors (PEBBLEs) and fiber optic microsensors for oxygen sensing

[+] Author Affiliations
Zoe Chen-Esterlit, Serban F. Peteu, Heather A. Clark, William McDonald, Raoul Kopelman

Univ. of Michigan (USA)

Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, 156 (May 3, 1999); doi:10.1117/12.347517
Text Size: A A A
From Conference Volume 3602

  • Advances in Fluorescence Sensing Technology IV
  • Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson
  • San Jose, CA | January 23, 1999

abstract

In this paper we report the use of phase sensitive fluorometry to obtain preliminary results from opto-chemical fluorescent oxygen nanosensors. PEBBLE (Probe Encapsulated By Biologically Localized Embedding) sensors were fabricated by immobilizing tris(4,7-diphenyl-1,10-phenanthroline)Ru(II) chloride and tris(1,10-phenanthroline)Ru(II) chloride within a polyacrylamide matrix. PEBBLEs have diameters of 20 - 200 nm and exhibit excellent performance for dissolved oxygen detection. Their performance is compared with micrometer-sized (10 - 20 micrometer) optical fiber sensors and free dye in solution. Oxygen sensing ability of PEBBLEs was tested in the presence of other quenchers and compared with free dyes in solution. While PEBBLEs have been developed for minimally invasive intracellular chemical analysis they show additional advantages, such as increased dynamic range, compared to microsensors, and an absence of interference (quenching) by heavy ions in contrast to free dye solutions.

© (1999) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Zoe Chen-Esterlit ; Serban F. Peteu ; Heather A. Clark ; William McDonald and Raoul Kopelman
"Comparative study of optical fluorescent nanosensors (PEBBLEs) and fiber optic microsensors for oxygen sensing", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, 156 (May 3, 1999); doi:10.1117/12.347517; http://dx.doi.org/10.1117/12.347517


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

 

 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.