Tissue imaging using two-photon video rate microscopy

Ki Hean Kim; Christof Buehler; Chen-Yuan Dong; Barry R. Masters; Peter T. C. So

doi:10.1117/12.349216

1 June 1999 Tissue imaging using two-photon video rate microscopy

Ki Hean Kim, Christof Buehler, Chen-Yuan Dong, Barry R. Masters, Peter T. C. So

Proceedings Volume 3604, Optical Diagnostics of Living Cells II; (1999) https://doi.org/10.1117/12.349216
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Non-invasive optical diagnosis of cellular and extracellular structure and biochemistry in thick tissue is becoming a reality with the maturation of the two-photon imaging. Today, the slow imaging speed of typical two-photon microscopes is a major hurdle in realizing their clinical potential. We have developed a high-speed two-photon microscope optimized for acquiring 3-D tissue images in real time. The scanning speed improvement of this system is obtained by the use of an air bearing polygonal mirror. The maximum achievable scanning rate is 40 microseconds per line, which is about 100 times faster than conventional scanning microscopes. High-resolution fluorescence images were recorded in real-time by an intensified CCD camera. Using this instrument, we have monitored the movements of protozoas and mapped the collagen/elastin fiber structures in excised human skin.

Citation Download Citation

Ki Hean Kim, Christof Buehler, Chen-Yuan Dong, Barry R. Masters, and Peter T. C. So "Tissue imaging using two-photon video rate microscopy", Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); https://doi.org/10.1117/12.349216

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