Scanning near-field optical/atomic-force microscope (SNOAM) for biomedical applications

Eiichi Tamiya; Shinichiro Iwabuchi; Atsuko Hashigasako; Yuji Murakami; Toshifumi Sakaguchi; Yasutaka Morita; Kenji Yokoyama

doi:10.1117/12.350635

17 June 1999 Scanning near-field optical/atomic-force microscope (SNOAM) for biomedical applications

Eiichi Tamiya, Shinichiro Iwabuchi, Atsuko Hashigasako, Yuji Murakami, Toshifumi Sakaguchi, Yasutaka Morita, Kenji Yokoyama

Author Affiliations +

Proceedings Volume 3607, Scanning and Force Microscopies for Biomedical Applications; (1999) https://doi.org/10.1117/12.350635
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

A scanning near-field optical/atomic-force microscope (SNOAM) system was applied to simultaneous topographic and fluorescence imaging of biological samples in air and liquid. The SNOAM uses a bent optical fiber simultaneously as a dynamic mode atomic-force microscopy cantilever and a scanning near-field optical microscopy probe. The SNOAM system used 458 or 488 nm from Ar ion laser multiline of excitation of green fluorescent protein (GFP), since a native GFP has been known to give a maximum at 395 nm and a broad absorption spectrum until 500 nm. Topographic and fluorescence images of recombinant E.coli were obtained simultaneously with a high spatial resolution which was apparently better than that of a conventional confocal microscope.

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Eiichi Tamiya, Shinichiro Iwabuchi, Atsuko Hashigasako, Yuji Murakami, Toshifumi Sakaguchi, Yasutaka Morita, and Kenji Yokoyama "Scanning near-field optical/atomic-force microscope (SNOAM) for biomedical applications", Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999); https://doi.org/10.1117/12.350635

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