Human coronary atherosclerosis studied in vitro by catheter-based transluminal Raman spectroscopy

Hendrik P. J. Buschman; Tjeerd J. Romer; Michael L. Wach; Eric Marple; Arnoud van der Laarse; Albert V.G. Bruschke; Gerwin J. Puppels

doi:10.1117/12.345409

9 April 1999 Human coronary atherosclerosis studied in vitro by catheter-based transluminal Raman spectroscopy

Hendrik P. J. Buschman, Tjeerd J. Romer, Michael L. Wach, Eric Marple, Arnoud van der Laarse, Albert V.G. Bruschke, Gerwin J. Puppels

Author Affiliations +

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345409
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We used Raman spectroscopy to assess the chemical composition and pathological states of atherosclerosis in in vitro intact human coronary artery tissue. Human coronary artery samples expressing different stages of atherosclerosis were mounted in an in vitro set-up and perfused with a salt solution. NIR laser light was delivered to the tissue through the central fiber of an optical fiber catheter that was inserted transluminally into a buffer- perfused arterial segment. Tissue Raman signal was collected by seven fibers surrounding the central fiber. The collected Raman light was launched into a spectrometer and imaged onto a CCD. High signal to noise, low background tissue Raman spectra were obtained in 10-60 s form artery samples. The spectral information from each collection fiber was linearly modeled with a Raman spectral model that quantifies the chemical composition of the arterial wall. The model results showed excellent fits to all Raman spectra. A diagnostic algorithm, that has proven to have excellent correlation with historilogical classification by a pathologist, classified the examined tissue into one of three pathological states. From these experiments we conclude that intravascular optical fiber Raman spectroscopy can provide in situ histopathology, which may be used to study vascular disease in vivo.

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Hendrik P. J. Buschman, Tjeerd J. Romer, Michael L. Wach, Eric Marple, Arnoud van der Laarse, Albert V.G. Bruschke, and Gerwin J. Puppels "Human coronary atherosclerosis studied in vitro by catheter-based transluminal Raman spectroscopy", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345409

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