Light-induced fluorescence of human colon tissue: dependence on histological and histochemical properties studied by means of a simplified model for simulation

Renato Marchesini; Sabrina Fumagalli; Emanuele Pignoli; Adele Emilia Sichirollo; Stefano Tomatis; Silvana Di Palma; Marco Dal Fante; Pasquale Spinelli; Anna Cleta Croce; Giovanni F. Bottiroli

doi:10.1117/12.198704

4 January 1995 Light-induced fluorescence of human colon tissue: dependence on histological and histochemical properties studied by means of a simplified model for simulation

Renato Marchesini, Sabrina Fumagalli, Emanuele Pignoli, Adele Emilia Sichirollo, Stefano Tomatis, Silvana Di Palma, Marco Dal Fante, Pasquale Spinelli, Anna Cleta Croce, Giovanni F. Bottiroli

Author Affiliations +

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198704
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

In an attempt to evaluate the role of the histological and histochemical features of adenocarcinoma and normal tissue on their fluorescence spectral patterns, we developed a Monte Carlo model to simulate the fluorescence emission from tissue. According to histology, normal colon and adenocarcinoma were considered composed of three and two tissue layers, respectively (i.e., superficial epithelium, mucosa and submucosa; tumor mass and submucosa). Characterization of the fluorescence pattern of the tissue layers as well as of several histological components was performed by spectrofluorometric analysis on ex vivo samples. Results obtained from differently modelling the morphology and histological arrangement of colon suggest the following remarks. The decrease in fluorescence intensity observed in passing from normal to abnormal tissue could be ascribed mainly to a modification in morphology. When carcinomatous tissue is involved a modification in the spectral shape is also expected.

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Renato Marchesini, Sabrina Fumagalli, Emanuele Pignoli, Adele Emilia Sichirollo, Stefano Tomatis, Silvana Di Palma, Marco Dal Fante, Pasquale Spinelli, Anna Cleta Croce, and Giovanni F. Bottiroli "Light-induced fluorescence of human colon tissue: dependence on histological and histochemical properties studied by means of a simplified model for simulation", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198704

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